Diabetic rats had significantly impaired behavioural and cognitive functions. Administration of high doses of fluoride f can alter glucose homeostasis and lead to insulin resistance ir. Proteomic analysis of gastrocnemius muscle in rats with. Podocalyxin is a sialoprotein critical to maintaining integrity of filtration. Effects of stzinduced diabetes on the relative weights of. Preclinical evaluation of antihyperglycemic activity of clerodendron infortunatum leaf against streptozotocininduced diabetic rats, diabetes therapy, 2011, pp. Rapamycin protects against hepatic injury in streptozotocininduced. Therefore, four groups of rats were allocated as control c, stzinduced dm d, and. Diabetic rats 200 mgdl were divided in 7 groups 3 rats per group and maintained in separate cages for 5 h, 10 h, 24 h, 36 h, 48 h, 5 days and 10 days after stz injection. In diabetic rats, the number and size of islets was reduced and the immunoreactivity for insulin was diminished. Effects of stzinduced diabetes on the relative weights of kidney, liver and pancreas in albino rats.
The most frequently used intravenous dose of this drug to induce diabetes in rats is 65 mgkg b. Biochemical effects of oleuropein in streptozotocin induced diabetic male rat. Iron overload is frequently observed in type 2 diabetes mellitus dm2, but the underlying mechanisms remain unclear. Background diabetes mellitus is an endocrine metabolic disorder of impaired carbohydrate, fat and protein metabolisms characterized by chronic hyperglycemia. Brain indoleamines in alloxan and streptozotocininduced diabetic rats. Male wistar rats 60 days old were randomly distributed into two groups of 18 animals. Limiting prolonged inflammation during proliferation and. Antidiabetic properties and mechanism of action of orthosiphon stamineus enth bioactive subfraction in streptozotocininduced diabetic rats. Streptozotocininduced diabetic ratsmice stz model advantages. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of c. Diabetes mellitus was induced in male spraguedawley rats weighing 200220 g by an injection of streptozotocin stz dissolved in a citrate buffer into the tail vein at a dose of 45 mgkg.
This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocininduced diabetes that were chronically exposed to f. Antihyperglycemic effect of an extract of myrtus communis in streptozotocininduced diabetes in mice. Streptozotocininduced diabetic rats either were given unrestricted access to a caseinbased diet expt. Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. Streptozotocin use in mice and rats version 1 date of publication.
Diabetes mellitus was induced by single intraperitoneal injection of 35. Diabetes was induced by streptozotocin 40 mgkg in female wistar rats. Streptozotocin stz is toxic to pancreatic islet insulinproducing bcells, thus inducing diabetes. Hyperglycemia enhances the production of reactive oxygen species, leading. Evaluation of antidiabetic activity of biologically. Sbt on the plasma blood glucose and cholesterol level in diabetic rats.
Alhagi maurorum camel thorn plant is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. Challenges and issues with streptozotocininduced diabetes. Streptozotocininduced diabetic rat model background strain. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Diabetic nephropathy is an emergent issue in china with increase in patients with type ii diabetes. The aim of this study was to assess the behaviour in streptozotocininduced diabetic wistar rats and to evaluate the behavioural effects of caffeine administration. Preclinical evaluation of antihyperglycemic activity of. Detailed in this unit are protocols for producing stz. Diabetes is associated with declining sexual function in women. Challenges and issues with streptozotocininduced diabetes a clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics author links open overlay panel sameer n. Streptozotocininduced diabetic models in mice and rats.
The sizeselective permeability of different vascular beds to a group of fluorescein isothiocyanate dextrans. Evaluation of antidiabetic activity of ipomoea aquatica. Wistar rat, male, 250g body weight streptozotocin stz induction protocol. There are several successful chinese herbal products for the treatment of patients with diabetic nephropathy in china. At a dose of 500 mgkg, raw ginger was significantly effective in lowering serum glucose, cholesterol and triacylglycerol levels in the gingertreated diabetic rats compared with the control diabetic rats. The stzinjected rats exhibited hyperglycaemia accompanied with weight loss, indicating their diabetic condition. Consumption of water and food, volume of urine, serum glucose increases in diabetic animals in comparison with normal rats but the levels of serum insulin, cpeptide and body weight decreases. The onset speed of hyperglycemia is important to the. Streptozotocin stz is an antibiotic that produces pancreatic islet. Diabetes is a complex metabolic disorder involving many organs and can devastate the lives of affected individuals. Does honey have any salutary effect against streptozotocin induced diabetes in rats.
The effect of rosuvastatin and simvastatin therapy 10 mgkg for 5 weeks on bloodbrain barrier bbb, bloodretinal barrier brb, and cardiac muscle permeability of streptozotocininduced diabetic rats was studied. Several studies have reported that some parts of the c. Antidiabetic activity of different extracts of myrtus communis in. Statins may have favorable effects on endothelial barrier function. Effect of bauhinia holophylla treatment in streptozotocininduced. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin stzinduced diabetic rats and suggested. Other models of diabetes include genetic models and the use of a prolonged highfat diet regimen.
Antihyperglycemic and antihyperlipidemic activities of. We hypothesize that hepcidin may be directly regulated by insulin and play an important role in iron overload in dm2. Antidiabetic activity of different extracts of myrtus. Described in this unit are two protocols used to produce stzinduced diabetes in mice basicprotocol1andratsbasicprotocol2. The aim of this study was to investigate potential hypoglycemic and hypolipidemic effects of protodioscin in diabetic rats.
Statins ameliorate endothelial barrier permeability. The mechanism of alloxan and streptozotocin action in b. The stz model provides a good, simple screening tool to test compounds and to further understand the complex development of neuropathic pain. The present study was intended to provide an insight into the specific effects of progesterone, the other female sex hormone, in the diabetic renal complications. In this study, we used an argonbased round atmosphericpressure plasma jet appj for enhancing wound healing in streptozotocin stz induced diabetic rats. The onset speed of hyperglycemia is important to the development of neuropathic hyperalgesia in streptozotocin. Antidiabetic and hypolipidaemic properties of ginger. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for hyperglycemia and alzheimers in a large dose, as well as. Regulation of podocalyxin expression in the kidney of. Gynura procumbens, an herb with known anti diabetic properties, was used in this investigation to determine the effect of its aqueous and ethanolic extracts on sperm quality and testosterone level of male streptozotocininduced type 1 diabetic rats. Quercetin derivatives in onions have been regarded as the most important flavonoids to improve diabetic status in cells and animal models.
Streptozotocin enters the b cell via glucose transporter glut 2 and causes. Gynura procumbens, a medicinal plant locally known as sambung nyawa, is widely used traditionally in south east asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Novel insight into streptozotocininduced diabetic rats. Request pdf streptozotocininduced diabetic models in mice and rats streptozotocin stz is an antibiotic that can cause pancreatic. The present study was aimed to examine the hypoglycemic and insulinsensitizing capacity of onion peel extract ope containing high quercetin in high fat dietstreptozotocininduced diabetic rats and to elucidate the mechanism of its insulin. Comparison of streptozotocin and alloxaninduced diabetes. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Diabetes mellitus is one of the major health problems all over the world. Traditional plant treatment for diabetes has shown a surging interest in the last few decades. In control and streptozotocintreated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Biochemical effects of oleuropein in streptozotocin.
Histopathological and immunohistochemical study of antidiabetic. It also changes normal metabolism in diabetic rats in comparison with normal rats. Adult female wistar rats were divided into four groups n 6group. The streptozotocin diabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. In this study, we examined the effects of hippophae rhamnoides l. After 4 weeks of feeding on a highfat diet, rats were injected intraperitoneally with streptozotocin to. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. If the inline pdf is not rendering correctly, you can download the pdf file here.
Streptozotocininduced experimental diabetes in male. Streptozotocininduced diabetic neuropathy in mice and rats. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of queensland. Metformin and glibenclamide were used as control drugs in this study. However, the mechanisms mediating the biological activity of these products are still unclear. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. Objective the present study was designed to evaluate the diabetes induced neuronal damage in the hippocampus of the rat brain. Does honey have any salutary effect against streptozotocin.
Human islets are considerably more resistant to alloxan than those of the rat and mouse eizirik et al. Intravenous injection of 60mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell. Streptozotocininduced diabetes in the rat is associated. Pdf effect of streptozotocin induced diabetes on rat. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. Wound healing in streptozotocininduced diabetic rats. Pdf brain indoleamines in alloxan and streptozotocin. Anti diabetic effect of sorghum extract on hepatic gluconeogenesis of streptozotocininduced diabetic rats by jungmin kim and yongsoon park download pdf 693 kb. Onion peel extracts ameliorate hyperglycemia and insulin. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. Diabetes was induced in young, weaned, agematched rat pups by streptozotocin.
Induction of diabetes by streptozotocin in rats springerlink. Currently, stz is most often used to induce diabetes in rats and mice. We therefore examined the hepatic iron content, serum iron parameters, intestinal iron absorption, and liver hepcidin expression in rats treated with. Histology and immunohistochemistry of the pancreatic islets of langerhans in control left panel and streptozotocininduced diabetic right panel rats. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. Serum and liver lipids distributions in streptozotocin induced. This study tested the hypothesis that dietary arginine supplementation increases endothelial tetrahydrobiopterin bh 4 availability for nitric oxide no synthesis in diabetic rats. Results of in vitro and in vivo analyses in rats treated with leaf extract or agnps show that both the extract and the biologically synthesized agnps have antidiabetic activity. Dietary larginine supplementation enhances endothelial. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes.
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